Lipid Hydroperoxide (LPO) is a kind of peroxide produced by unsaturated fatty acid chain through
the action of free radicals or reactive oxygen species. Under pathological conditions, the enhancement of
lipid peroxidation can lead to the increase of LPO with low content. The increase of LPO content will
damage the structure and function of cells. LPO content is closely related to the immune system and aging.
LPO is heated under acidic conditions to produce malondialdehyde (MDA). MDA is condensed with
thiobarbituric acid (TBA) to produce a brownish red substance named 3,5,5-trimethyloxazole-2,4-dione.
Its maximum absorption wavelength is 532nm. The content of LPO in the sample can be estimated after
colorimetry.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, Desk centrifuge, Adjustable Transferpettor, Water Bath, Micro Quartz Cuvette/96
Well Flat-Bottom UV Plate, Mortar/Homogenizer, Ice, Distilled Water.