The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene (HCRTR1) encodes a G-protein coupled receptor that selectively binds orexin A. [provided by RefSeq, Jan 2009]
Function:
Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade.
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
O43614
Gene ID:
3061
Database links:
Entrez Gene: 3061 Human
Entrez Gene: 230777 Mouse
Entrez Gene: 25593 Rat
Omim: 602392 Human
SwissProt: O43613 Human
SwissProt: P58307 Mouse
SwissProt: P56718 Rat
Unigene: 388226 Human
Unigene: 246595 Mouse
Unigene: 88262 Rat
Entrez Gene: 3062 Human
Entrez Gene: 387285 Mouse
Entrez Gene: 25605 Rat
Omim: 602393 Human
SwissProt: O43614 Human
SwissProt: P58308 Mouse
SwissProt: P56719 Rat
Unigene: 151624 Human
Unigene: 699147 Human
Unigene: 335300 Mouse
Unigene: 9893 Rat
食欲素受体(OXR)Orexin receptor—是一种G蛋白偶合受体. 下丘脑是调节饮食及能量平衡的中枢.学者认为：在下丘脑可促进食欲且来源于同一前体的两种新神经肽:食欲素A和B,它们可激活两种密切相关且与G蛋白偶联的细胞表面受体(OX1R,OX2R).含Orexin的神经细胞在下丘脑腹外侧呈对称的不连贯分布. 在饮食反馈调节中具有重要作用.食欲素与其它影响进食的神经肽之间存在错综复杂的关系.它在增加摄食、饮水、调节睡眠觉醒周期、生殖、体温、血压和感觉等方面有广泛作用.
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Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
Primary: Anti- Orexin receptor 1+2 (SL1095R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Cerebellum tissue lysates
Primary: Anti-Orexin receptor 1+2 (SL1095R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse Cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Orexin receptor 1+2) Polyclonal Antibody, Unconjugated (SL1095R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Orexin receptor 1+2) Polyclonal Antibody, Unconjugated (SL1095R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CD200R/Orexin receptor Polyclonal Antibody, Unconjugated(SL1095R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CD200R/Orexin receptor Polyclonal Antibody, Unconjugated(SL1095R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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