Rabbit Anti-ROBLD3/FITC Conjugated antibody

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ENDAP; Endosomal adaptor protein p14; HSPC003; LAMTOR2; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2; Late endosomal/lysosomal Mp1 interacting protein; Late endosomal/lysosomal Mp1-interacting protein; LTOR2_HUMAN; MAPBPIP; MAPK

Cat:

SL13705R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Dog,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human ROBLD3

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

13kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

background:
MP1 (MEK partner 1) functions as a scaffolding protein in the mitogen activated protein (MAP) kinase signaling pathway. Growth factor induced MAP kinase activation is selectively mediated by the extracellular signal-regulated kinase (ERK) cascade. MAPBPIP (mitogen-activated protein-binding protein-interacting protein), also known as p14 and late endosomal/lysosomal MP1-interacting protein, functions as an adaptor protein augmenting the regulation of the MAP kinase cascade. Partner proteins MAPBPIP and MP1 are structurally almost identical each with a five-stranded ∫-sheet flanked between a two-helix and one-helix layer. MAPBPIP compels the recruitment of MP1 to late endosomes where they form a very stable heterodimeric complex required for ERK activation on endosomes. Knockdown of the individual proteins in the MP1/MAPBPIP complex resulted in decreased expression of the partner proteins which implies greater stability of the heterodimeric complex than either MP1 or MAPBPIP individually. Early research suggests the MP1-MAPBPIP-MEK-1 signaling complex may be critical in the regulation of tissue homeostasis.

Function:
As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal SLVATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.

Subcellular Location:
Late endosome membrane. Lysosome membrane.

Tissue Specificity:
Defects in LAMTOR2 are the cause of immunodeficiency due to defect in MAPBP-interacting protein (ID-MAPBPIP) [MIM:610798]. This form of primary immunodeficiency syndrome includes congenital neutropenia, partial albinism, short stature and B-cell and cytotoxic T-cell deficiency.

Similarity:
Belongs to the GAMAD family.

Database links:

Entrez Gene: 28956 Human

Omim: 610389 Human

SwissProt: Q9Y2Q5 Human

Unigene: 632483 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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