Mouse Anti-human CD90/Thy-1 antibody

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CD90 / Thy1; CD7; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen;

Cat:

SLM30105M

Species Reactivity：

(predicted: Human,)

Immunogen：

HEL cells

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Concentration：

0.5mg/ml

Clonality：

Monoclonal

Isotype：

Mouse IgG1

Applications：

IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Blank control:HepG2. Primary Antibody (green line): Mouse Anti- CD90 antibody (SLM30105M) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Mouse IgG . Secondary Antibody : Goat anti-Mouse IgG-CY5Dilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $376.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Function:
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity:
Abundant in lymphoid tissues.

Post-translational modifications:
Glycosylation is tissue specific. Sialylation of N-glycans at Asn-93 in brain and at Asn-42, Asn-93 and Asn-117 in thymus.

Similarity:
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
P04216

Gene ID:
7070

Database links:

Entrez Gene: 7070 Human

Entrez Gene: 21838 Mouse

Entrez Gene: 24832 Rat

Omim: 188230 Human

SwissProt: P04216 Human

SwissProt: P01831 Mouse

SwissProt: P01830 Rat

Unigene: 644697 Human

Unigene: 3951 Mouse

Unigene: 108198 Rat

Picture

Blank control:HepG2.
Primary Antibody (green line): Mouse Anti- CD90 antibody (SLM30105M)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Mouse IgG .
Secondary Antibody : Goat anti-Mouse IgG-CY5
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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