Mouse Anti-T7 tag antibody | Sunlong Medical

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T7-tag; Major capsid protein 10A; MASMTGGQQMG epitope tag; Minor capsid protein 10B; T7 epitope tag.

Cat:

SLM33012M

Species Reactivity：

Recombinant protein

Immunogen：

KLH conjugated synthetic peptide T7-tag:MASMTGGQQMG

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:1000-5000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Lane 1: T7-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ugLane 2: T7-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ugPrimary: Anti-T7-Tag (SLM33012M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 41 kDObserved band size: 45 kD

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Unit:

Price: $196.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The T7 tag is commonly engineered onto the N- or SLC terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems.

SWISS:
N/A

Gene ID:
N/A

Picture

Sample:
Lane 1: T7-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ug
Lane 2: T7-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ug
Primary: Anti-T7-Tag (SLM33012M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD

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