Polyamine Oxidase (PAO) is a key enzyme which catalyse the aerobic degradation of polyamines.
Polyamines have strong binding capacity to nucleic acid, protein and cell membrane molecules and
directly affect cell growth, differentiation and apoptosis. PAO could be involved in the response to stress
and development of plant by regulating polyamines content.
PAO catalyzes polyamines to produce H2O2. Peroxidase catalyzes the production of H2O2 into oxygen
and oxidizes o-dianisidine to form colored substance which has light absorption at 500nm. The color depth
is linear with PAO activity.
Reagents and Equipment Required but Not Provided:
Spectrophotometer/Microplate reader, balance, constant temperature foster box/water-bath,
centrifuge,
micro
glass
cuvette/96-well
flat-bottom
plate,
adjustable
transferpettor,
mortar/homogenizer/cell ultrasonic crusher, ice and distilled water.