GOT is widely found in animals, plants, microbes and cultured cells. It catalyzes the reversal of amino
reactions and is an important enzyme in amino acid metabolism. In addition, GOT is the highest in
cardiomyocytes and is commonly used as an assisted examination of myocardial infarction and
myocarditis in clinical practice. The serum concentration of liver damage can also be increased.
GOT catalyze α-ketoglutaric acid react with aspartate to produce glutamic acid and oxaloacetic acid.
Oxaloacetic acid is further decarboxylated to form pyruvate, pyruvate can react with 2, 4-
dinitrophenylhydrazine to produce 2,4-dinitrophenylhydrazone, which shows brownish red in alkaline
condition, the activity of GOT enzyme activity can be calculated by measuring the absorbance of 505 nm.
Reagent and Equipments Required but not provided:
Spectrophotometer/microplate reader, water bath, desk centrifuge, transferpettor, micro glass cuvette/96
well flat-bottom plate, mortar/homogenizer, ice and distilled water.
