Rabbit Anti-APOBEC3B antibody

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APOBEC1L; Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3B; apolipoprotein B mRNA editing enzyme, catalytic;ARCD3; ARP4; bK150C2.2; Cytidine deaminase; DJ742C19.2; FLJ21201; Phorbolin 1 related protein; Phorbolin 2; Phorbolin 2/3; Phorbo

Cat:

SL12494R

Species Reactivity：

Rat,(predicted: Human,)

Immunogen：

KLH conjugated synthetic peptide derived from human APOBEC3B:1-100/382

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:jurkat(human)cell Lysate at 40 ugPrimary: Anti-APOBEC3B (SL12494R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 46kDObserved band size: 46 kDTissue/cell: Rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated(SL12494R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated(SL12494R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated(SL12494R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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APOBEC proteins inhibit retroviruses by deaminating cytosine residues of viral RNA and DNA. The seven APOBEC3 genes or pseudogenes are found in a cluster thought to result from gene duplication on chromosome 22. APOBEC3 proteins are thought to be RNA editing enzymes and have roles in cell growth. APOBEC3B, also known as phorbolin-1-related protein or phorbolin-2/3, is a 382 amino acid protein belonging to the cytidine and deoxycytidylate deaminase family. APOBEC3B lacks cytidine deaminase activity on RNA molecules, but has been shown to bind to apoB and AU-rich RNAs. APOBEC3B forms a homodimer and interacts with APOBEC3G. APOBEC3B is expressed at at moderate levels in heart, testis, thymus, prostate, ovary, spleen and peripheral blood leukocytes.

Function:
APOBEC3B is a member of the cytidine deaminase gene family. However, it lacks cytidine deaminase activity, at least on RNA molecules (monomeric nucleoside substrates or synthetic apoB, NF1 and NAT1 RNA template). It binds to apoB and AU-rich RNAs. It is unable to reduce HISLV1 infectivity in vitro.

Subunit:
Homodimer. Interacts with APOBEC3G. Does not interact with APOBEC1.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed at high and moderate levels in peripheral blood leukocytes, spleen, testes, heart, thymus, prostate and ovary. Also expressed at low levels in several other tissues.

Similarity:
It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22.
Belongs to the cytidine and deoxycytidylate deaminase family.
Contains 2 CMP/dCMP deaminase zinc-binding domains

SWISS:
Q9UH17

Gene ID:
9582

Database links:

Entrez Gene: 9582 Human

Entrez Gene: 315137 Rat

Omim: 607110 Human

SwissProt: Q9UH17 Human

SwissProt: P60705 Rat

Unigene: 226307 Human

Unigene: 658626 Human

Unigene: 2936 Rat

Picture

Tissue/cell: Rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated(SL12494R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated(SL12494R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: Mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated(SL12494R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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