Rabbit Anti-GTPBP9 antibody

Home > Product > Antibody > Rabbit Anti-GTPBP9 antibody

DNA damage-regulated overexpressed in cancer 45 protein; DOC45; GBP45; GTP-binding protein 9 (putative); GTP-binding protein 9; GTP-binding protein PTD004; homologous yeast-44.2 protein; Obg like ATPase 1; Obg-like ATPase 1; OLA1; OLA1_HUMAN; PTD004.

Cat:

SL9554R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Cow,Sheep,Fruit Fly,)

Immunogen：

KLH conjugated synthetic peptide derived from human GTPBP9:151-250/396

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Raji Cell (Human) Lysate at 30 ugPrimary: Anti-GTPBP9 (SL9554R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 45kDObserved band size: 50kDSample: Brain (Mouse) Lysate at 40 ugPrimary: Anti-GTPBP9 (SL9554R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 45kDObserved band size: 50kDSample: HepG2 Cell (Human) Lysate at 30 ugTT Cell (Human) Lysate at 30 ugPrimary: Anti-GTPBP9 (SL9554R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 45kDObserved band size: 50kDBlank control (Black line):Molt4 (Black). Primary Antibody (green line): Rabbit Anti-GTPBP9 antibody (SL9554R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

GTP-binding protein 9 is a 396 amino acid protein that belongs to the Obg-related GTPase family under the translation factors (TRAFAC) class. Originally thought to only have GTPase activity, Obg-related GTPase family members have been shown to also have ATPase activity. In Homo sapians, GTPBP9 exhibits a preference for binding ATP over GTP, with GTP binding occuring only at high nucleotide concentration. One cause for ATP affinity and GTP discrimination is thought to be a substitution of glutamine for a hydrophobic amino acid in Obg-related family members; this is the same substitution that inactivates Ras-like GTPases. GTPBP9 contains a SLCterminal TGS domain that binds to ligands and an N-terminal G domain which binds nucleotides. GTPBP9 is expressed as three isoforms produced by alternative splicing.

Function:
Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.

Subunit:
Monomer.

Subcellular Location:
Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Predominantly cytoplasmic, shuttles between the nucleus and the cytoplasm.

Tissue Specificity:
Expressed in all tissues tested but its expression is more abundant in testis, liver, lung, and brain. Overexpressed in several malignancies, including cancers of the colon, rectum, ovary, lung, stomach, and uterus.

Similarity:
Belongs to the GTP1/OBG family.
Contains 1 G (guanine nucleotide-binding) domain.

SWISS:
Q9NTK5

Gene ID:
29789

Database links:

Entrez Gene: 29789 Human

Entrez Gene: 67059 Mouse

Entrez Gene: 296488 Rat

Omim: 611175 Human

SwissProt: Q9NTK5 Human

SwissProt: Q9CZ30 Mouse

SwissProt: A0JPJ7 Rat

Unigene: 157351 Human

Unigene: 22661 Mouse

Unigene: 5874 Rat

Picture

Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-GTPBP9 (SL9554R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD

Sample:
HepG2 Cell (Human) Lysate at 30 ug
TT Cell (Human) Lysate at 30 ug
Primary: Anti-GTPBP9 (SL9554R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD

Blank control (Black line):Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-GTPBP9 antibody (SL9554R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall