Rabbit Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) antibody

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LATS1 +LATS2 (phospho T1079 + T1041); Serine/threonine protein kinase LATS2; KPM; Large tumor supressor, homolog 1; LATS, large tumor suppressor, homolog 1 (Drosophila); LATS, large tumor suppressor, homolog 2 (Drosophila); LATS1 +LATS2 (phospho T1079 + T

Cat:

SL7913R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human LATS1 around the phosphorylation site of Thr1079:EF(P-T)FR

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) (SL7913R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 124 kDObserved band size: 124 kDSample: Hela(Human) Cell Lysate at 30 ugPrimary: Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) (SL7913R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 124 kDObserved band size: 124 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-LATS1+LATS2 (Thr1079 +Thr1041)) Polyclonal Antibody, Unconjugated (SL7913R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. phosphopeptidenon phosphopeptide

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor.

Function:
Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.

Subunit:
Complexes with CDK1 in early mitosis. LATS1-associated CDK1 has no mitotic cyclin partner and no apparent kinase activity. Binds phosphorylated ZYX, locating this protein to the mitotic spindle and suggesting a role for actin regulatory proteins during mitosis. Binds to and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis. Interacts (via PPxY motif 2) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA.

Subcellular Location:
Cytoplasm, cytoskeleton, centrosome. Note=Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.

Tissue Specificity:
Expressed in all adult tissues examined except for lung and kidney.

Post-translational modifications:
Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGSLCkinase SLCterminal domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.

SWISS:
O95835

Gene ID:
26524

Database links:

Entrez Gene: 26524 Human

Entrez Gene: 9113 Human

Entrez Gene: 16798 Mouse

Entrez Gene: 50523 Mouse

Omim: 603473 Human

Omim: 604861 Human

SwissProt: O95835 Human

SwissProt: Q9NRM7 Human

SwissProt: Q7TSJ6 Mouse

SwissProt: Q8BYR2 Mouse

Picture

Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) (SL7913R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-LATS1+LATS2 (Thr1079 +Thr1041)) Polyclonal Antibody, Unconjugated (SL7913R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

phosphopeptide non phosphopeptide

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Specific References (8) | SL7913R has been referenced in 8 publications.

[IF=10.317] Qi Yang. et al. A novel biodegradable external stent regulates vein graft remodeling via the Hippo-YAP and mTOR signaling pathways. Biomaterials. 2020 Nov;258:120254 WB ; Rat.

PubMed:32805499

[IF=4.6] Fatah,et al.Reduced Proximal Tubular Expression of Megalin and Neonatal Fc Receptor in Proteinuria: The Role of Matrix Metalloproteinases.(2017) .Fatah, H., Benfaed, N., Chana, R. S., Chunara, M. H., Barratt, J., Baines, R. J., & Brunskill, N. J. (2017). Reduced proximal tubular expression of protein endocytic receptors in proteinuria is associated with urinary receptor shedding. Nephrology Dialysis Transplantation, 33(6), 934–943. doi:10.1093/ndt/gfx321 IHSLCP + WB ; Human.

PubMed:doi:10.1093/ndt/gfx321

[IF=3.468] Konishi,et al.Proliferation of hepatic stellate cells, mediated by YAP and TAZ, contributes to liver repair and regeneration after liver ischemia-reperfusion injury.(2018) American Journal of Physiology. Gastrointestinal and Liver Physiology. 314:G471-G482. WB ; Mouse.

PubMed:29351389

[IF=3.65] Grijalva, James, et al. "Dynamic Alterations in Hippo Signaling Pathway and YAP Activation during Liver Regeneration." American Journal of Physiology-Gastrointestinal and Liver Physiology (2014). WB ; Rat.

PubMed:24875096

[IF=8.579] Lixia Chen. et al. CSRP2 suppresses colorectal cancer progression via p130Cas/Rac1 axis-meditated ERK, PAK, and HIPPO signaling pathways.. Theranostics. 2020; 10(24): 11063–11079 WB ; Human.

PubMed:33042270

[IF=4.237] Wang C et al. MicroRNA‐10a promotes epithelial‐to‐mesenchymal transition and stemness maintenance of pancreatic cancer stem cells via upregulating the Hippo signaling pathway through WWC2 inhibition. J Cell Biochem. 2020 Nov;121(11):4505-4521. WB ; Human.

PubMed:32542845

[IF=4.237] Wang C et al. MicroRNA-10a promotes epithelial-to-mesenchymal transition and stemness maintenance of pancreatic cancer stem cells via upregulating the Hippo signaling pathway through WWC2 inhibition. J Cell Biochem . 2020 Nov;121(11):4505-4521. WB ; Human.

PubMed:32542845

[IF=5.589] Gao R et al. PM2.5-associated nitro-PAH exposure promotes tumor cell metastasis through Hippo-YAPmediated transcriptional regulation. Sci Total Environ. 2019 Aug 15;678:611-617. WB ; Human.

PubMed:31078851