Home > Product > Antibody > Rabbit Anti-RSRC2 antibody
1500011J06Rik; arginine/serine-rich coiled-coil 2; FLJ11021; RSRC2_HUMAN.
Cat:
SL6097R
Species Reactivity:
Human,Mouse,(predicted: Rat,Chicken,Dog,Pig,Cow,Horse,)
Immunogen:
KLH conjugated synthetic peptide derived from human RSRC2:351-434/434
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: Large intestine (Mouse) Lysate at 40 ugPrimary: Anti-RSRC2 (SL6097R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kDSample: Spleen (Mouse) Lysate at 40 ugStomach (Mouse) Lysate at 40 ugPrimary: Anti- RSRC2 (SL6097R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 52 kDParaformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RSRC2) Polyclonal Antibody, Unconjugated (SL6097R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RSRC2) Polyclonal Antibody, Unconjugated (SL6097R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Unit:
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Product PDFs
Datasheet:


RSRC2 (arginine/serine-rich coiled-coil 2) possibly plays a role in cell proliferation and may be a novel tumor suppressor of esophageal cancer cell growth.

Similarity:
Belongs to the RSRC2 family.

SWISS:
Q7L4I2

Gene ID:
65117

Database links:

Entrez Gene: 65117 Human

Entrez Gene: 208606 Mouse

Entrez Gene: 72807 Rat

SwissProt: Q7L4I2 Human

SwissProt: A2RTL5 Mouse

SwissProt: Q5PQR4 Rat

Unigene: 432996 Human



Picture

Sample:
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-RSRC2 (SL6097R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Stomach (Mouse) Lysate at 40 ug
Primary: Anti- RSRC2 (SL6097R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RSRC2) Polyclonal Antibody, Unconjugated (SL6097R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RSRC2) Polyclonal Antibody, Unconjugated (SL6097R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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