Rabbit Anti-ICAM1 antibody | Sunlong Medical

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BB2; CD54 antigen; CD54; ICAM1; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2;

Cat:

SL4615R

Species Reactivity：

Human,Mouse,(predicted: Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human CD54/ICAM-1:451-505/532<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=1μg/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela(Human) Cell Lysate at 30 ugRaji(Human) Cell Lysate at 30 ugPrimary: Anti-ICAM1 (SL4615R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 56 kDObserved band size: 61 kDSample: Lane 1: A431 (Human) Cell Lysate at 30 ugLane 2: Huvec (Human) Cell Lysate at 30 ugLane 3: HepG2 (Human) Cell Lysate at 30 ugLane 4: Siha (Human) Cell Lysate at 30 ugLane 5: U937 (Human) Cell Lysate at 30 ugPrimary: Anti-ICAM1 (SL4615R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 58/100 kDObserved band size: 58 kDSample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti-ICAM1 (SL4615R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 56 kDObserved band size: 56 kDSample: Lung (Mouse) Lysate at 40 ugSpleen (Mouse) Lysate at 40 ugLymph node (Mouse) Lysate at 40 ugHeart (Mouse) Lysate at 40 ugA549(Human) Cell Lysate at 40 ugA431(Human) Cell Lysate at 40 ugU937(Human) Cell Lysate at 40 ugK562(Human) Cell Lysate at 40 ugPrimary: Anti- ICAM1 (SL4615R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 56 kDObserved band size: 56 kDBlank control(blue): U937(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-ICAM1 antibody(SL4615R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008].

Function:
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Subunit:
Homodimer (Probable). Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Binds to coxsackievirus A21 capsid proteins and acts as a receptor for this virus.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Post-translational modifications:
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

Similarity:
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
P05362

Gene ID:
3383

Database links:

Entrez Gene: 3383 Human

Entrez Gene: 15894 Mouse

Entrez Gene: 25464 Rat

Omim: 147168 Human

Unigene: 643447 Human

SwissProt: P05362 Human

SwissProt: P13597 Mouse

SwissProt: Q00238 Rat

Unigene: 435508 Mouse

Unigene: 12 Rat

Picture

Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: Huvec (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: Siha (Human) Cell Lysate at 30 ug
Lane 5: U937 (Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1 (SL4615R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58/100 kD
Observed band size: 58 kD

Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1 (SL4615R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Sample:
Lung (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
A549(Human) Cell Lysate at 40 ug
A431(Human) Cell Lysate at 40 ug
U937(Human) Cell Lysate at 40 ug
K562(Human) Cell Lysate at 40 ug
Primary: Anti- ICAM1 (SL4615R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-ICAM1 antibody(SL4615R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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