Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-RelB (SL4185R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
Sample:
Raji Cell (Human) Lysate at 30 ug
Primary: Anti-RelB (SL4185R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RelB) Polyclonal Antibody, Unconjugated (SL4185R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample: mouse blood
primary antibody: Anti-RelB/PE-Cy5(SL4185R-PE-Cy5).
concentration:1:20, 4℃ incubate 30min.
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