Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of Myosin have been cloned: smooth muscle Myosins, striated muscle Myosins and non-muscle Myosins . Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle.
Subunit:
Myosin is a hexamer of 2 heavy chains and 4 light chains.
Tissue Specificity:
Expressed in fetal and adult skeletal muscle.
Similarity:
Contains 3 EF-hand domains.
SWISS:
Q96A32
Gene ID:
29895
Database links:
Entrez Gene: 29895 Human
Entrez Gene: 396470 Chicken
Entrez Gene: 317657 Cow
Entrez Gene: 4632 Human
Entrez Gene: 17901 Mouse
Entrez Gene: 17907 Mouse
Entrez Gene: 24584 Rat
Entrez Gene: 56781 Rat
Omim: 160780 Human
SwissProt: P02609 Chicken
SwissProt: P05976 Human
SwissProt: P06741 Human
SwissProt: Q14324 Human
SwissProt: P05978 Mouse
SwissProt: P97457 Mouse
SwissProt: P02602 Rabbit
SwissProt: P02603 Rabbit
SwissProt: P02600 Rat
SwissProt: P04466 Rat
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Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Rat Muscle tissue lysates
Primary: Anti-MYLPF (SL5159R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kDa
Observed band size: 15 kDa
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-MYLPF (SL5159R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 19 kD
Sample:
A673(Human) Cell Lysate at 30 ug
Primary: Anti-MYLPF (SL5159R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 19 kD
Tissue/cell: muscle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Fast skeletal Myosin Polyclonal Antibody, Unconjugated(SL5159R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYLPF) Polyclonal Antibody, Unconjugated (SL5159R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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