Sample:
Lane 1: Mouse Lymph node tissue lysates
Lane 2: Mouse Lung tissue lysates
Lane 3: Mouse Placenta tissue lysates
Lane 4: Rat Lymph node tissue lysates
Lane 5: Rat Placenta tissue lysates
Lane 6: Human Raji cell lysates
Primary: Anti-Lyn (SL2906R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 50 kDa
Sample:
SP2/0(Mouse) Cell Lysate at 30 ug
Primary: Anti-Lyn (SL2906R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Lyn Polyclonal Antibody, Unconjugated(SL2906R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: human glioma tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Lyn Polyclonal Antibody, PE conjugated (SL2906R-PE) 1:200, used at 1:200 dilution for 60 minutes at 37°C. Excitation wavelength: 488nm, 555nm; Emission wavelength: 578nm
Overlay histogram showing Mouse spleen cells stained with SL2906-PE (red line). The cells were fixed with 1% paraformaldehyde (10 min).The cells were then incubated with the antibody (SL2906R-PE, 2ug/1x106cells) for 30 min at 22-25°C. Isotype control antibody (black line) was rabbit IgG (2ug/1x106cells) used under the same conditions. Acquisition of events were used for analysis.
|