Rabbit Anti-OCLN antibody | Sunlong Medical

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Occludin, Occludin-1; Occludin1; Occludin 1; FLJ08163; BLCPMG; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; OCLN; OCLN_HUMAN; Tight junction protein occludin.

Cat:

SL1495R

Species Reactivity：

Human,(predicted: Mouse,Rat,Dog,Pig,Cow,Horse,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse OCLN:431-522/522<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=2µg/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: A549 Cell Lysate at 30 ugPrimary: Anti- OCLN (SL1495R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 57 kDObserved band size: 57 kDSample: Lane 1: A549 (Human) Cell Lysate at 30 ugLane 2: MCF-7 (Human) Cell Lysate at 30 ugLane 3: A431 (Human) Cell Lysate at 30 ugLane 4: HepG2 (Human) Cell Lysate at 30 ugLane 5: Hela (Human) Cell Lysate at 30 ugPrimary: Anti-OCLN (SL1495R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 65/55/38/28 kDObserved band size: 65/38/28 kDSample: Kidney (Mouse) Lysate at 40 ugPrimary: Anti-OCLN (SL1495R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 57 kDObserved band size: 57 kDSample: A549 Cell Lysate at 30 ugPrimary: Anti- OCLN (SL1495R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 57 kDObserved band size: 60 kDBlank control: MCF7. Primary Antibody (green line): Rabbit Anti-OCLN antibody (SL1495R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.The blue histogram is unstained cells (293FT cells). The Wathet Blue histogram is cells stained with secondary antibody alone.The Orange histogram is cells stained with rabbit IgG isotype control antibodyplus secondary antibody. The green histogram is cells stained with Rabbit Anti-OCLN antibody (SL1495R) plus secondary antibody (Concentraion 1: 20).

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq].

Function:
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Subunit:
Interacts with TJP1/ZO1 and with VAPA.

Subcellular Location:
Membrane; Multi-pass membrane protein. Cell junction, tight junction.

Tissue Specificity:
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. May be phosphorylated by PKC during translocation to cell-cell contacts.

Similarity:
Belongs to the ELL/occludin family.
Contains 1 MARVEL domain.

SWISS:
Q61146

Gene ID:
18260

Database links:

Entrez Gene: 18260 Mouse

Entrez Gene: 100506658 Human

Entrez Gene: 397236 Pig

Entrez Gene: 83497 Rat

Entrez Gene: 403844 Dog

Omim: 602876 Human

SwissProt: Q28269 Dog

SwissProt: Q16625 Human

SwissProt: Q61146 Mouse

SwissProt: Q6P6T5 Rat

Unigene: 592605 Human

Unigene: 967 Mouse

Unigene: 31429 Rat

Picture

Sample: A549 Cell Lysate at 30 ug
Primary: Anti- OCLN (SL1495R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD

Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: MCF-7 (Human) Cell Lysate at 30 ug
Lane 3: A431 (Human) Cell Lysate at 30 ug
Lane 4: HepG2 (Human) Cell Lysate at 30 ug
Lane 5: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-OCLN (SL1495R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65/55/38/28 kD
Observed band size: 65/38/28 kD

Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-OCLN (SL1495R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD

Sample: A549 Cell Lysate at 30 ug
Primary: Anti- OCLN (SL1495R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 60 kD

Blank control: MCF7.
Primary Antibody (green line): Rabbit Anti-OCLN antibody (SL1495R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

The blue histogram is unstained cells (293FT cells).
The Wathet Blue histogram is cells stained with secondary antibody alone.
The Orange histogram is cells stained with rabbit IgG isotype control antibody plus secondary antibody.
The green histogram is cells stained with Rabbit Anti-OCLN antibody (SL1495R) plus secondary antibody (Concentraion 1: 20).

Product Feedback Wall

Specific References (17) | SL1495R has been referenced in 17 publications.

[IF=2.984] WB, IF ; Pig.

PubMed:32717305

[IF=2.73] Sun, Yan‐Na, et al. "Effects of insulin combined with idebenone on blood–brain barrier permeability in diabetic rats." Journal of Neuroscience Research (2014). WB ; Rat.

PubMed:25421718

[IF=2.984] Ruirui Luoet al. Clostridium perfringens beta2 toxin induced in vitro oxidative damage and its toxic assessment in porcine small intestinal epithelial cell lines. Gene . 2020 Oct 30;759:144999. IF ; pig.

PubMed:32717305

[IF=3.454] Li T et al. Bovine α-lactalbumin hydrolysates ameliorate obesity-associated endotoxemia and inflammation in high-fat diet-fed mice through modulation of gut microbiota. Food Funct. 2019 May 17. WB ; Mouse.

PubMed:31099356

[IF=3.47] Li T et al. Milk fat globule membrane supplementation modulates the gut microbiota and attenuates metabolic endotoxemia in high-fat diet-fed mice. Journal of Functional Foods,2018 47, 56–65. WB ; Mouse.

PubMed:10.1016/j.jff.2018.05.038

[IF=4.12] Zou et al. Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response. (2016) Sci.Rep. 6:39299 IHC ; Mouse.

PubMed:27982094

[IF=2.86] Zhao, Yuan, et al. "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets."International journal of molecular sciences 12.12 (2011): 8502-8512. WB, IHSLCP ; Pig.

PubMed:22272087

[IF=2.03] Gu, Yan-ting, et al. "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." Neuroscience letters 493.3 (2011): 140-144. WB ; Rat.

PubMed:21334421

[IF=15.923] Changsheng Xing. et al. Microbiota regulate innate immune signaling and protective immunity against cancer. Cell Host Microbe. 2021 Apr;: IHC ; Mouse.

PubMed:33894128

[IF=3.545] Lackner I et al. Toll-Like Receptor-Mediated Cardiac Injury during Experimental Sepsis. Mediators of Inflammation, 2020, 1–12. ICF ; Mouse.

PubMed:doi:10.1155/2020/6051983

[IF=0] Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106. IHSLCP ; Human.

PubMed:Doi:10.5606/Tr-ENT.2019.64936

[IF=2.776] Weber B et al. Lung injury after asphyxia and hemorrhagic shock in newborn piglets: Analysis of structural and inflammatory changes. PLoS One. 2019 Jul 5;14(7):e0219211. IHF ; Piglet.

PubMed:31276543

[IF=3.743] Li N et al. Modification effects of SanWei GanJiang Powder on liver and intestinal damage through reversing bile acid homeostasis. Biomed Pharmacother. 2019 Aug;116:109044. IHSLCP ; Mouse.

PubMed:31174088

[IF=4.122] Hayes CL.et al. Commensal microbiota induces colonic barrier structure and functions that contribute to homeostasis. (2018)Scientific Reports, 8(1). IF ; Mouse.

PubMed:30242285

[IF=2.71] Guo, Xiaoling, et al. "Matrigel and Activin A promote cell-cell contact and anti-apoptotic activity in cultured human retinal pigment epithelium cells." Experimental Eye Research (2016). IF(ICC) ; Human.

PubMed:27130547

[IF=2.46] Zhao, Yuan, et al. "β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPESLCJ2." International Journal of Molecular Sciences 15.2 (2014): 1915-1926. Pig.

PubMed:24473141

[IF=2.46] Pan, Li, et al. "Effects of Soybean Agglutinin on Mechanical Barrier Function and Tight Junction Protein Expression in Intestinal Epithelial Cells from Piglets." International Journal of Molecular Sciences 14.11 (2013): 21689-21704. Pig.

PubMed:24189218