Rabbit Anti-phospho-FSCN1 (Ser39) antibody

Home > Product > Antibody > Rabbit Anti-phospho-FSCN1 (Ser39) antibody

Fascin (phospho Ser39); Fascin (phospho S39); 55 kDa actin bundling protein; Actin bundling protein; FAN 1; FAN1; Fascin 1; Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus); Fascin homolog 1; Fascin1; FLJ38511; FSCN 1; FSCN1; HSN; p

Cat:

SL0772R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human FSCN1 around the phosphorylation site of Ser39:AS(p-S)LK

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: U87MG(Human) Cell Lysate at 30 ugPrimary: Anti-phospho-FSCN1(Ser39) (SL0772R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDSample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Testis tissue lysates Lane 3: Mouse Spleen tissue lysates Lane 4: Rat Cerebrum tissue lysates Lane 5: Rat Testis tissue lysates Lane 6: Rat Spleen tissue lysates Lane 7: Human HepG2 cell lysates Lane 8: Human K562 cell lysates Lane 9: Human SH-SY5Y cell lysates Lane 10: Human U-2os cell lysatesPrimary: Anti- phospho-FSCN1 (Ser39) (SL0772R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDaObserved band size: 51 kDaParaformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FSCN1(Ser39)) Polyclonal Antibody, Unconjugated (SL0772R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: musle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-phospho-FSCN1(Ser39) Polyclonal Antibody, Unconjugated(SL0772R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingA549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (phospho-FSCN1 (Ser39)) polyclonal Antibody, Unconjugated (SL0772R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Blank control: U937(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 3μl in 100 μL1X PBS containing 0.5% BSA(green).

prev next

Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Human fascin is a highly conserved actin-bundling protein. Fascin, encoded by the human homolog for sn (hsn) gene, has been localized to microspikes and stress fibers of cultured cells where it is thought to be involved in the formation of microfilament bundles. It is expressed predominantly in dendritic cells. Lymphoid cells, myeloid cells and plasma cells are negative. However, Reed Sternberg cells in Hodgkin’s lymphoma are positive for fascin staining. Epstein-Barr virus may induce expression of fascin in B cells.

Function:
Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.

Subcellular Location:
Cytoplasm > cytoskeleton. Cell projection > filopodium. Cell projection > invadopodium.

Tissue Specificity:
Ubiquitous.

Post-translational modifications:
Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin.

Similarity:
Belongs to the fascin family.

SWISS:
Q16658

Gene ID:
6624

Database links:

Entrez Gene: 6624 Human

Entrez Gene: 14086 Mouse

Entrez Gene: 683788 Rat

Omim: 602689 Human

SwissProt: Q16658 Human

SwissProt: Q61553 Mouse

SwissProt: P85845 Rat

Unigene: 111680 Human

Unigene: 289707 Mouse

Unigene: 199526 Rat

肌动蛋白集束蛋白/圆线虫紫癜抗体。
FSCN1为细胞骨架肌动蛋白的一种，该蛋白有蛋白结合,桥连、肌动蛋白丝结合的功能。主要参与细胞增殖、肌动蛋白细胞骨架组织和生物发生、肌动蛋白丝束形成。< Picture

Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Mouse Spleen tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Rat Testis tissue lysates
Lane 6: Rat Spleen tissue lysates
Lane 7: Human HepG2 cell lysates
Lane 8: Human K562 cell lysates
Lane 9: Human SH-SY5Y cell lysates
Lane 10: Human U-2os cell lysates
Primary: Anti- phospho-FSCN1 (Ser39) (SL0772R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 51 kDa

Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FSCN1(Ser39)) Polyclonal Antibody, Unconjugated (SL0772R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: musle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-phospho-FSCN1(Ser39) Polyclonal Antibody, Unconjugated(SL0772R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (phospho-FSCN1 (Ser39)) polyclonal Antibody, Unconjugated (SL0772R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Blank control: U937(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 3μl in 100 μL1X PBS containing 0.5% BSA(green).

Product Feedback Wall